cd185 (cxcr5) antibody, anti-human, reafinity (Miltenyi Biotec)

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Miltenyi Biotec cd185 (cxcr5) antibody, anti-human, reafinity
Cd185 (Cxcr5) Antibody, Anti Human, Reafinity, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 93/100, based on 31 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cxcr5/CD185+(CXCR5)+Antibody%2C+anti-human%2C+REAfinity/custom%40130-122-325%4042314934
Average 93 stars, based on 31 article reviews

cd185 (cxcr5) antibody, anti-human, reafinity - by Bioz Stars, 2026-11

93/100 stars

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Staining:

Article Title: Orf virus-based vectors induce potent germinal center B cell, Tfh cell, and CD8 + T cell responses.
Article Snippet: Additionally, we added BD Horizon brilliant stain buffer (BD Biosciences, 566385) whenever more than one brilliant violet dye was used in the staining mix. .. Antibodies against CD45R/B220 (RA3-6B2), Bcl-6 (K112-91), CD127 (A7R34), CD138 (281-2), CD19 (6D5), CD38 (REA616), CD4 (RM4-5), CD44 (IM7), CD45.1 (A20), CD45.2 (104), CD62L (MEL-14), CD8α (53-6.7), CXCR5 (L138D7), GL7 antigen (GL7), IgD (11-26c.2a), IgM (IL/41), Irf-4 (3E4), KLRG1 (2F1/KLRG1), and PD-1 (29F.1A12) were used for staining and purchased from BD, Miltenyi, BioLegend, and ThermoFisher Scientific (eBiosciences, Invitrogen). ..

Article Title: Preserved efficacy of lyophilized SARS-CoV-2 mRNA vaccine incorporating novel ionizable lipids after one year at 25 °C.
Article Snippet: Acquisition was performed using a Gallios flow cytometer (Beckman Coulter). .. Tfh cells were defined as CD45R-CD4+, CD44high, CXCR5+, PD-1+, and the following antibodies were used for surface staining: CD45R (B220)APC-Vio770 (Miltenyi, 130-110-849), CD4-VioBright FITC (Miltenyi, 130-118-692), CD44-PerCP-Vio700 (Miltenyi, 130-128-625), CD185 (CXCR5)-APC (Miltenyi, 130-119-129), and CD279 (PD1)-PE (Miltenyi, 130-111-953). .. The SARS-CoV-2 hCoV19/USA/ CA-Stanford-109_S21/2022 (Lineage XBB; Omicron Variant) (GISAID: EPI_ISL_15509864) was isolated from a human on October 10, 2022, in California and was obtained from BEI resources (NR-58925).

Article Title: Influenza vaccine-specific CD4 + T cell responses are impaired in older adults.
Article Snippet: After 1 h, protein transport was blocked using GolgiPlug (1 μL/mL, BD Biosciences) and GolgiStop (0.7 μL/mL, BD Biosciences), and cells were cultured for a further 12h. .. Cells were washed and stained with directly conjugated antibodies specific for CCR7 (clone G043H7, BioLegend), CD3 (clone REA613, Miltenyi Biotec), CD4 (clone REA623, Miltenyi Biotec), CD8 (clone REA734, Miltenyi Biotec), CD45RA (clone REA1047, Miltenyi Biotec), and CXCR5 (clone RF8B2, BD Biosciences). .. Nonviable events were excluded using a LIVE/DEAD Fixable Aqua Dead Cell Stain Kit (Thermo Fisher Scientific).

Recombinase Polymerase Amplification:

Article Title:
Article Snippet: .. Research Resource Identifiers (RRIDs) were obtained from The Antibody Registry (antibodyregistry.org): HLA-DR (Cat# 564040, BD Biosciences RRID:AB_2738558, Clone G46-6, 1:250) CD8 (Cat# 612942, Becton Dickinson, RRID:AB_2870223, Clone RPA-T8, 1:1000) CD27 (Cat# 741833, Becton Dickinson, RRID:AB_2871168, Clone M-T271, 1:500) CD4 (Cat# 612887, Becton Dickinson, RRID:AB_2870176, Clone SK3, 1:250) CD45RA (Cat# 566114, Becton Dickinson, RRID:AB_2739516, Clone HI100, 1:1000) CD57 (Cat# 393304, Biolegend, RRID:AB_2728425, Clone QA17A04, 1:1000) CD62L (Cat# 563808, Becton Dickinson, RRID:AB_2738433, Clone DREG-56, 1:500) TIM3 (Cat# 565566, Becton Dickinson, RRID:AB_2744370, Clone 7D3, 1:1000) CD25 (Cat# 563701, Becton Dickinson, RRID:AB_2744338, Clone M-A251, 1:500) CFSE (Cat# C34554, ThermoFisher, 1:1000) TIGIT (Cat# 46-9500-42, Invitrogen, RRID:AB_10853679, Clone MBSA43, 1:1000) CCR7 (Cat# 353236, Biolegend, RRID:AB_2563640, Clone G043H7, 1:1000) PD-1 (Cat# 561272, Becton Dickinson, RRID:AB_10611585, Clone N/A, 1:1000) LAG3 (Cat# 369304, Biolegend, RRID:AB_2566480, Clone 11C3C65, 1:500) FVS780 (Cat# 565388, Becton Dickinson, 1:1000) Jovi1 (Cat# ANC-101-030, Ancell, lone JOVI-1, 1:1000) Humanized Jovi 1 and variants were in house generated Anti-idiotype for Jovi-1 was in house generated aCD3 (Cat# 317334, Biolegend, RRID:AB_2561452, Clone OKT3, 1:1000) Streptavidin (Cat# 405243, Biolegend, 1:1000) Anti-CD34 (Cat# 343607, Biolegend, RRID:AB_2074356, Clone 561, 1:200) anti TCR alpha/beta Monoclonal Antibody (WT31) PE conjugated (Cat# 12-9955-42, eBioscience, RRID:AB_10596819, Clone WT31, 1:1000) Goat anti-Human IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa FluorTM 647 (Cat# A-21445, Invitrogen, RRID:AB_2535862, Clone N/A, 1:1000) Goat anti-Mouse IgG2a Cross-Adsorbed Secondary Antibody, Alexa FluorTM 488 (Cat# A-21131, Invitrogen, RRID:AB_2535771, Clone N/A, 1:1000) HuAnti-CD34 (Cat# FAB7227A, R&D System, RRID:AB_10972777, Clone QBend10, 1:200) Anti CD3 (Milteny, Cat# 130-113-142, RRID:AB_2725970, Clone REA613, 1:500) Anti CD45RA (Mylteny, Cat# 130-117-747, RRID:AB_2733126, Clone REA1047, 1:500) Anti CCR7 (Miltenyi, Cat# 130-119-583, RRID:AB_2751741, Clone REA546, 1:500) Anti CXCR5 (Miltenyi, Cat# 130-122-795, RRID:AB_2811386, Clone REA103, 1:500) Anti TIM-3 (Miltenyi, Cat# 130-121-334, RRID:AB_2784165, Clone REA635, 1:500) Validation The antibodies used in this study are commercially available and were used for the applications validated by manufacturers. ..

Generated:

Biomarker Discovery:

Isolation:

Article Title: CXCR5 Signals Fine-Tune Dendritic Cell Transcription and Regulate T H 2 Development
Article Snippet: .. Briefly, msLN cells isolated from Hp -infected B6 and Cxcr5 −/− msLNs at 8 days p.i. were positively enriched using anti-CD11c beads (Miltenyi) in the presence of anti-CD16/32 (2.4G2 BioXCell). .. Purified OT-II.4get transgenic CD4 + T cells (2 × 10 5 ) were incubated with the MACS-enriched DC populations (4 × 10 4 ) in complete media in the presence of increasing concentrations of OVA (323–339) peptide (New England Peptide) for 4 days with or without 100 ng recombinant murine CHI3L1 protein (R&D Systems).

Article Title: CXCR5 Signals Fine-Tune Dendritic Cell Transcription and Regulate T H 2 Development.
Article Snippet: .. Briefly, msLN cells isolated from Hp-infected B6 and Cxcr5−/− msLNs at 8 days p.i. were positively enriched using anti-CD11c beads (Miltenyi) in the presence of anti-CD16/32 (2.4G2 BioXCell). .. Purified OT-II.4get transgenic CD4+ T cells (2 × 105) were incubated with the MACS-enriched DC populations (4 × 104) in complete media in the presence of increasing concentrations of OVA(323–339) peptide (New England Peptide) for 4 days with or without 100 ng recombinant murine CHI3L1 protein (R&D Systems).

Article Title: CXCR5 Signals Fine-Tune Dendritic Cell Transcription and Regulate T H 2 Development.
Article Snippet: Expression of the IL-4 reporter (GFP) in the live CD4+ (7AAD−CD4+) OT-II cells was detected by flow cytometry using a BD FACSCantoTM, (BD Biosciences, San Jose, CA, USA). .. DCs were positively selected with Miltenyi CD11c beads from spleens isolated from uninfected Cxcr5−/− and CD11c-Cxcr5Tg mice after mechanical disruption and RBC lysis. ..

Article Title: CXCR5 Signals Fine-Tune Dendritic Cell Transcription and Regulate T H 2 Development
Article Snippet: Expression of the IL-4 reporter (GFP) in the live CD4 + (7AAD − CD4 + ) OT-II cells was detected by flow cytometry using a BD FACSCanto TM , (BD Biosciences, San Jose, CA, USA). .. DCs were positively selected with Miltenyi CD11c beads from spleens isolated from uninfected Cxcr5 −/− and CD11c - Cxcr5 Tg mice after mechanical disruption and RBC lysis. ..

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cd185 (cxcr5) antibody, anti-human, reafinity (Miltenyi Biotec)

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Staining:

Recombinase Polymerase Amplification:

Generated:

Biomarker Discovery:

Isolation:

Infection:

Disruption:

Red Blood Cell Lysis:

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